Winners of Nikon’s 47th annual Small World Photomicrography competition

Nikon Instruments recently announced the winners and finalists for its 47th annual Small World Photomicrography competition. Thousands of images were submitted from around the world. A panel of judges including Hank Green, Dr. Nsikan Akpan of New York Public Radio, and Dr. Hesper Rego from Yale School of Medicine selected the pool of 100 winners and honorable mentions.

The First Place prize was awarded to Jason Kirk. Kirk captured around 200 images of a southern live oak leaf’s trichromes using a custom-made microscope system that combines color filtered transmitted light with diffused reflected light. He then stacked them to produce the final result.

‘The lighting side of it was complicated,’ he reveals. ‘Microscope objectives are small and have a very shallow depth of focus. I couldn’t just stick a giant light next to the microscope and have the lighting be directional. It would be like trying to light the head of a pin with a light source that’s the size of your head. Nearly impossible.’ Jason admits he edited the color temperature and hue in post-production to help illustrate the different elements in the image.

Nikon Small World was created to show how the world of art and science unite under a microscope. All winning and honorable mentions can be viewed in the competition’s website.

1st Place: Jason Kirk

School: Baylor College of Medicine

Department: Optical Imaging & Vital Microscopy Core

Location: Houston, Texas, USA

About the Image: Trichome (white appendages) and stomata (purple pores) on a southern live oak leaf.

Techique: Image Stacking

Magnification: 60X (Objective Lens Magnification)

2nd Place: Esmeralda Paric & Holly Stefen

School: Dementia Research Centre, Macquarie University

Department: Biomedical Sciences

Location: Macquarie Park, NSW, Australia

About the Image: A microfluidic device containing 300k networking neurons in 2 isolated populations. Both sides were treated with a unique virus and bridged by axons.

Technique: Fluorescence

Magnification: 40X (Objective Lens Magnification)

3rd Place: Frank Reiser

School: Nassau Community College

Department: Biology

Location: Garden City, New York, USA

About the Image: Rear leg, claw, and respiratory trachea of a louse (Haematopinus suis).

Technique: Darkfield, Image Stacking

Magnification: 5X (Objective Lens Magnification)

4th Place: Paula Diaz

School: MinusPain, Pontificia Universidad Catolica de Chile

Department: Physiology

Location: Santiago, Region Metropolitana, Chile

About this Image: Sensory neuron from an embryonic rat.

Technique: Fluorescence

Magnification: 10X (Objective Lens Magnification)

7th Place: Dr. Tong Zhang & Dr. Paul Stoodley

School: The Ohio State University

Department: Campus Microscopy & Imaging Facility

Location: Columbus, Ohio, USA

About the Image: Head of a tick.

Technique: Confocal

Magnification: 10X (Objective Lens Magnification)

8th Place: Dr. Amy Engevik

School: Medical University of South Carolina

Department: Regenerative Medicine and Cell Biology

Location: Charleston, South Carolina, USA

About the Image: Cross section of mouse intestine

Technique: Fluorescence

Magnification: 10X (Objective Lens Magnification)

10th Place: Sébastien Malo

Location: Saint Lys, Haute – Garonne, France

About the Image: Vein and scales on a butterfly wing (Morpho didius)

Technique: Image Stacking, Reflected Light

Magnification: 20X (Objective Lens Magnification)

12th Place: Jakub Sumbal

School: Masaryk University

Department: Histology and Embryology

Location: Brno, Czech Republic

About the Image: Breast organoid showing contractile myoepithelial cells (blue) crawling on secretory breast cells (red).

Technique: Confocal

Magnification: 40X (Objective Lens Magnification)

13th Place: Dr. Felice Placenti

Specialty: FP Nature and Landscape Photography

Location: Siracusa, Sicilia, Italy

About this Image: Cotton fabric with pollen grains.

Technique: Darkfield, Image Stacking

Magnification: 10X (Objective Lens Magnification)

14th Place: Dr. Joern N. Hopke

Location: Waban, Massachusetts, USA

About this Image: Snowflake

Technique: Image Stacking

Magnification: 4X (Objective Lens Magnification)

17th Place: Martin Kaae Kristiansen

Location: Aalborg, Nordjylland, Denmark

About the Image: Filamentous strands of Nostoc cyanobacteria captured inside a gelatinous matrix.

Technique: Image Stacking, Polarized Light

Magnification: 4X (Objective Lens Magnification)

19th Place: Billie Hughes

Location: Bangkok, Thailand

About this Image: Calcite crystal inclusion suspended in a spinel gemstone.

Technique: Darkfield, Image Stacking

Magnification: 40X (Objective Lens Magnification)

20th Place: Alison Pollack

Location: San Anselmo, California, USA

About this Image: Slime mold (Arcyria pomiformis).

Technique: Image Stacking, Reflected Light

Magnification: 10X (Objective Lens Magnification)

Author: Go to Source